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LCA4BIO is a pioneering project aimed at developing and validating a new suite of enhanced, standardized, and precise methodologies tailored for assessing environmental impacts and circularity within bio-based systems.These methodologies will be instrumental in facilitating the green transition of the European industry by providing practical tools to evaluate the sustainability performance of bio-based products.LCA4BIO will address the development of prospective life cycle assessment methodologies, incorporating considerations for upscaling and future scenarios using Integrated Assessment Models with manageable levels of uncertainty.